Bio-Mediated Sandy Soil Stabilization Using Urease Enzymatic Calcite Precipitation: A Sustainable Solution
نویسندگان
چکیده
منابع مشابه
Urease activity in microbiologically-induced calcite precipitation.
The role of microbial urease in calcite precipitation was studied utilizing a recombinant Escherichia coli HB101 containing a plasmid, pBU11, that encodes Bacillus pasteurii urease. The calcite precipitation by E. coli HB101 (pBU11) was significant although its precipitation level was not as high as that by B. pasteurii. Addition of low concentrations (5-100 microM) of nickel, the cofactor of u...
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As one of the emerging soil improvement techniques, microbial induced calcite precipitation (MICP) can bond soil grains together and improve the engineering properties of soil [1]. bacteria produces urease enzyme can catalyze urea to carbonate and result in calcite precipitation in elevated pH environment. The forms of calcite precipitation around the bacteria limit the availability of oxygen a...
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The optimization of enzyme-mediated calcite precipitation was evaluated as a soil-improvement technique. In our previous works, purified urease was utilized to bio-catalyze the hydrolysis of urea, which causes the supplied Ca(2+) to precipitate with [Formula: see text] as calcium carbonate. In the present work, magnesium chloride was newly added to the injecting solutions to delay the reaction ...
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The enzyme urease drives the hydrolysis of urea leading to the release of ammonium ions and bicarbonate; in the presence of calcium, the rise in pH leads to increased calcium carbonate saturation and the subsequent precipitation of calcite. Although such alkalinizing ureolysis is widespread in nature, most studies have focussed on bacteria (i.e. indigenous communities or urease-active Sporosarc...
متن کاملMicrobiologically Induced Calcite Precipitation Mediated by Sporosarcina pasteurii.
The particular bacterium under investigation here (S. pasteurii) is unique in its ability, under the right conditions, to induce the hydrolysis of urea (ureolysis) in naturally occurring environments through secretion of an enzyme urease. This process of ureolysis, through a chain of chemical reactions, leads to the formation of calcium carbonate precipitates. This is known as Microbiologically...
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ژورنال
عنوان ژورنال: E3S Web of Conferences
سال: 2020
ISSN: 2267-1242
DOI: 10.1051/e3sconf/202018401113